Its applicability in human lives has been consequential, ranging from rapid urine analysis to detecting SARS-CoV-2 during the recent pandemic, under which the whole world travailed (Tai et al. Paper-based analytical devices (PADs) have been drawing significant attention for detecting analytes in biomedical, chemical, agricultural, and environmental fields since their initial demonstration by the Whitesides group (Martinez et al. RGB analysis of the colorimetric response showed a linear concentration range from 0 to 90 µM ( R 2 = 0.989) along with a detection limit of 28.375 µM. The viability of the fabricated platform has been assessed by colorimetric detection of glutathione using 3,3′,5,5′-tetramethylbenzidine–H 2O 2 redox system. Thus, wax stamped paper microplates can be used for a wide range of bioanalytical tests maneuvering reduced generation of non-biodegradable waste, minimal reagent usage, and inexpensive readout strategies. The technique further eradicates the requirement of customized stamps and the step of heating paper substrates for creating wax barriers. The method involves the use of commercially available polystyrene microplates as a stamp for wax patterning. In this study, a simple and rapid fabrication methodology for realizing a 96-well microtiter plate on paper has been developed. Wax printing has been a dominant technique to fabricate hydrophobic patterns on paper for fluid control, but the discontinuation of commercial solid ink printers has begun a genesis of alternate wax patterning strategies. Paper-based analytical devices have prominently emerged as a group of diagnostic tools with prospective to eliminate the expensive, time-consuming, and intricate analytical methodologies.

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